Leukemia Disease PDF Print E-mail
Friday, 23 December 2016 00:37

 Within the academic activities sequence for Biology Dept. / College/University of Baghdad, in its current cultural season 2016/2017, Dr. Fadil Mohammed Laftah ,specialist PhD at  cancer research Center/Newcastle University/United Kingdom presented the scientific tilted lecture to raise awarness of people:

Investigation for markers of DNA methylationto Predict Relapses of Acute Lymphocytic Leukemia (ALL) in Pediatrics

Abstract:

Acute Lymphocytic Leukemia disease regarded as the most widespread of cancer kinds in children , its rate more than 80% , also more than a half of the cases of (ALL) come from two genetic defects : hyper dipoydy (HeH) and ETV6-RUNX1 (t(12;21)

 Although these two genetic groups featured by a rate respond to chemotherapy, but a quite number of these affected kids suffering from relapsing this disease after stopping the medical chemotherapy, so checking the detecting markers of recurrence of the disease in these two genetic groups has contributed in the modification of the therapy program for these children to avoid its relapsing.

Variations considered in DNA methylationpatterns one of the distinctive features associated with the cancer initiation, and probably, these variations have the clinical importance as detection markers for predicating the development of the disease.

Our research study aimed to investigate the use of DNA methylation as detection marks for relapses of acute lymphocytic leukemia (ALL) in children. In this study two separated manners have been followed to study the detected disease markers in a sample consists of (189)child affected by this disease (the samples belong to the British Medical Research Council)) clinical trial 97/99).

1-    Analysis of DNA methylation for selected genetic sites (HOXA4, TWIST2, and TUSC3) by using the Pyrosequencing technology.

2-    Analysis of DNA methylation for a whole human genome by using 450 K BeadChip technologies.

In addition to the use of various diagnosed methylation patterns from the above mentioned manners (1,2) to determine the functional activity for some genes by using Lentiviral Vector for gene transfer to speeding or inhibitiontarget genes

The study got the following results:

1-    DNA methylation markers have not been determined to have a relation to detect relapsing of the (ALL) for kids in the studded sample whether by using selected special genes manner or by analyzing the DNA methylation for a whole genome.

2-    Limiting the association between special patterns of DNA methylation and undeveloped genetic groups for (ALL), and it is probably for these DNA methylation patterns to have a role in limiting the disease behavior and its developments.

3-    Determining the special DNA methylation patterns that have relation with immune & infection which is probably one of the causes for the disease initiation.

4-    The study was able from determining the variation of DNA methylation patterns for genes likely to have a relation with disease's initiation and its development:

-       Losing the methylation for TUSC3 gene in the most patients (79.5%) group ETV6-RUNX1

-       Restraining the gene expression for TUSC3 gene led to restrain the growth of cancer cells Reh which hold the above genetic disorder.

-       Increasing the induced genetic expression for HOXA4 gene resulted in excessing of the sensitivity in cancer Nalm6 cells for Cytarabine drug which is used to treat blood cancer diseases.  

 

 

 

 

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